The aim of this study is to examine the impact of career adaptability on ambidextrous behavior, explaining the role of entrepreneurial self-efficacy and entrepreneurial orientation. The statistical population of the study consists of all faculty members of Ferdowsi University of Mashhad. The sample size was determined to be 260 individuals using Cochran's formula, and participants were selected through stratified sampling. The required data were collected using standardized questionnaires, including the Entrepreneurial Orientation Questionnaire by Hughes and Morgan (2007), the Ambidextrous Behavior Questionnaire by Garcia (2016), the Career Adaptability Questionnaire by Savickas and Porfeli (2012), and the Entrepreneurial Self-Efficacy Questionnaire by De Noble (1999). The findings of the study indicate that entrepreneurial self-efficacy plays a moderating role in the relationship between career adaptability and ambidextrous behavior, and entrepreneurial orientation also serves as a moderator in this relationship.