This study investigates the effect of artificial intelligence opportunity perception on employee well-being considering the mediating role of informal learning and the moderating role of job insecurity perception. This applied, descriptive–survey study was conducted among 280 employees of the Iranian Graphic Designers Association in 2025, from which 188 participants were selected using simple random sampling. Data were collected via Xu et al.’s (2023) standardized questionnaire. Structural equation modeling was performed using SmartPLS 3 with descriptive analysis in SPSS 26. AI opportunity perception significantly predicted employee well-being (β=0.248, t=2.459) and informal learning (β=0.507, t=6.080). Informal learning significantly affected well-being (β=0.563, t=6.110). Sobel test confirmed the partial mediating role of informal learning (Z=3.758, p<0.001). Job insecurity perception did not significantly moderate the AI–informal learning relationship (t=1.322). Fostering opportunity-oriented perceptions of artificial intelligence enhances employee well-being primarily through strengthening informal learning processes, regardless of perceived job insecurity.